Objective: The purpose of this study was to investigate the effect of SPARK Physical Education (PE) program on fundamental motor skills in 4-6 year children. SPARK (Sports, Play, and Active Recreations for Kids) is an evidence based PE program designed in order to promote the lifelong wellbeing. Methods: In total, 90 children aged 4 to 6 years were selected randomly. The children were allocated into 3 groups with separate PE programs: 1-SPARK, 2-Gymnastics and 3-Routine activity. Using the Test of Gross Motor Development (TGMD-2), a pretest was done in all groups. Afterwards, SPARK and Gym PE programs were performed for 8 weeks and 3 sessions each week. The third group used to do the routine physical education program in their daycare. After 8 weeks (24 sessions), the post tests were done for all groups with the same scoring system as the pretest. Findings: The results showed that the SPARK programs had a higher efficacy on the promotion of the fundamental motor skills comparing to the routine physical education programs or gymnastics PE group. Conclusion: SPARK can be used as an appropriate alternative in order to promote the children's motor skills. (author abstract)