The importance of early intervention for developmental disabilities, such as Autism Spectrum Disorder, is increasingly recognized as critical to children’s later success, making it necessary to better understand how professionals who work regularly with young children view the use of developmental screening in their professional practice. This study examined knowledge and attitudes toward developmental screening practices among 130 early childcare and education providers. Survey methodology was used to collect and analyze information on providers’ perceptions of administering developmental screening, barriers to using screening in early childhood education settings, and differences in perceived barriers among providers with varying knowledge of autism spectrum disorder. (author abstract)